Recombinant E. coli alpha-Galactosidase A/GLA His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98992

Novus Biologicals, part of Bio-Techne
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NBP1-98992-50ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all alpha-Galactosidase A/GLA Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-451 of E.coli alpha-Galactosidase A/GLA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMMSAPKI TFIGAGSTIF VKNILGDVFH REALKTAHIA LMDIDPTRLE ESHIVVRKLM DSAGASGKIT CHTQQKEALE DADFVVVAFQ IGGYEPCTVT DFEVCKRHGL EQTIADTLGP GGIMRALRTI PHLWQICEDM TEVCPDATML NYVNPMAMNT WAMYARYPHI KQVGLCHSVQ GTAEELARDL NIDPATLRYR CAGINHMAFY LELERKTADG SYVNLYPELL AAYEAGQAPK PNIHGNTRCQ NIVRYEMFKK LGYFVTESSE HFAEYTPWFI KPGREDLIER YKVPLDEYPK RCVEQLANWH KELEEYKKAS RIDIKPSREY ASTIMNAIWT GEPSVIYGNV RNDGLIDNLP QGCCVEVACL VDANGIQPTK VGTLPSHLAA LMQTNINVQT LLTEAILTEN RDRVYHAAMM DPHTAAVLGI DEIYALVDDL IAAHGDWLPG WLHR

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli alpha-Galactosidase A/GLA His Protein

SDS-PAGE: Recombinant E. coli alpha-Galactosidase A/GLA His Protein [NBP1-98992]

SDS-PAGE: Recombinant E. coli alpha-Galactosidase A/GLA His Protein [NBP1-98992]

SDS-Page: Recombinant E. coli alpha-Galactosidase A/GLA Protein [NBP1-98992] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-98992
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Galactosidase A/GLA

melA(alpha-galactosidase) belongs to glycosyl hydrolase 4 family. Alpha-galactosidases catalyse the hydrolysis of saccharides containing o-1,6,-galactoside linkages. The three alpha-galactosidases catalyse the same reaction, but are localized in different cellular compartments: The E.coli enzyme is cytoplasmic protein and the human enzyme and the yeast enzyme is secretory proteins. Therefore, although the active enzyme from all three species has nearly the same molecular weight, structural similarities, as well as differences, are expected. Recombinant E.coil melA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Agalsidase alpha, GALA, Melibiase

Gene Symbol

GLA

Additional alpha-Galactosidase A/GLA Products

Product Documents for Recombinant E. coli alpha-Galactosidase A/GLA His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant E. coli alpha-Galactosidase A/GLA His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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