Recombinant Human Apolipoprotein A-I/ApoA1 Protein

Name: Recombinant Human Apolipoprotein A-I/ApoA1 Protein
Brand: Novus Biologicals
SKU: NBP2-34869

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34869

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Product Datasheet / COA

Catalog #

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P02647

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Apolipoprotein A-I/ApoA1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 243 amino acids corresponding to Apolipoprotein A-I/ApoA1 Source: E. coli

Uniprot ID: P02647

Amino Acid Sequence: DEPPQSPWDR VKDLATVYVD VLKDSGRDYV SQFEGSALGK QLNLKLLDNW DSVTSTFSKL REQLGPVTQE FWDNLEKETE GLRQEMSKDL EEVKAKVQPY LDDFQKKWQE EMELYRQKVE PLRAELQEGA RQKLHELQEK LSPLGEEMRD RARAHVDALR THLAPYSDEL RQRLAARLEA LKENGGARLA EYHAKATEHL STLSEKAKPA LEDLRQGLLP VLESFKVSFL SALEEYTKKL NTQ

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Apolipoprotein A-I/ApoA1 as determined by LAL method.

Predicted Molecular Mass

28.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Apolipoprotein A-I/ApoA1 Protein

SDS-PAGE: Recombinant Human Apolipoprotein A-I/ApoA1 Protein [NBP2-34869]

SDS-Page: Recombinant Human Apolipoprotein A-I/ApoA1 Protein [NBP2-34869]

Formulation, Preparation and Storage

NBP2-34869

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A-I encoded by the APOA1 gene is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Apolipoprotein A-I participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). Apolipoprotein A-I is also isolated as a prostacyclin (PGI2) stabilizing factor and thus may have an anticlotting effect. Apolipoprotein A-I has been shown to interact with ABCA1, GPLD1 and PLTP. Apolipoprotein A-I binds to lipopolysaccharide or endotoxin and has a major role in the anti-endotoxin function of HDL. Mature human ApoA-1 shares 65 % and 62 % a.a. sequence identity with murine and rat ApoA-1, respectively. It has an approximate molecular weight of 28.1 kDa.

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Gene Symbol

APOA1

UniProt