Recombinant Mouse Apolipoprotein A-I/ApoA1 His Protein

Name: Recombinant Mouse Apolipoprotein A-I/ApoA1 His Protein
Brand: Novus Biologicals
SKU: NBP2-52061

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52061

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Apolipoprotein A-I/ApoA1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-264 of Mouse APOA1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSDEPQSQW DKVKDFANVY VDAVKDSGRD YVSQFESSSL GQQLNLNLLE NWDTLGSTVS QLQERLGPLT RDFWDNLEKE TDWVRQEMNK DLEEVKQKVQ PYLDEFQKKW KEDVELYRQK VAPLGAELQE SARQKLQELQ GRLSPVAEEF RDRMRTHVDS LRTQLAPHSE QMRESLAQRL AELKSNPTLN EYHTRAKTHL KTLGEKARPA LEDLRHSLMP MLETLKTQVQ SVIDKASETL TAQ

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

30.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Apolipoprotein A-I/ApoA1 His Protein

SDS-PAGE: Recombinant Mouse Apolipoprotein A-I/ApoA1 His Protein [NBP2-52061]

SDS-Page: Recombinant Mouse Apolipoprotein A-I/ApoA1 Protein [NBP2-52061] - 15% SDS Page (3 ug)

Formulation, Preparation and Storage

NBP2-52061

Formulation	PBS (pH 7.4), 20% glycerol, 1 mM DTT
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Apolipoprotein A-I/ApoA1

Apolipoprotein A1 (apo A1) is the major polypeptide of the human plasma high density lipoprotein (HDL). The structure and function of the apo A1 gene are of interest because of the inverse correlation shown between HDL levels and coronary heart disease (1). The 267 amino acid precursor initially undergoes intracellular co-translational proteolytic cleavage into proapoA-I. ProapoA-I is secreted from the cell and was isolated from thoracic duct lymph in the apoA-I1 isoform position. Results indicate that apo A1 is present in human plasma, and undergoes post-translational proteolytic cleavage to mature plasma apoA-I (2). Cleavage of this protein with cyanogen bromide yields four fragments designated in the order of elution from Bio-Gel P-30 as CNBr I, II, III, and IV (3).

Alternate Names

Alp-1, APOA1, Apolipoprotein AI, Brp-14, Ltw-1, Lvtw-1, Sep-1, Sep-2

Gene Symbol

APOA1

Additional Apolipoprotein A-I/ApoA1 Products

Product Documents for Recombinant Mouse Apolipoprotein A-I/ApoA1 His Protein

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COA

Product Specific Notices for Recombinant Mouse Apolipoprotein A-I/ApoA1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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