AQUApure Tetra-Ub Chains (K11-linked) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # UC-45
Will be discontinued when existing inventory is gone.
Key Product Details
Product Specifications
Source
E. coli-derived human Tetra-Ubiquitin protein
Met1 - Gly76
Met1 - Gly76
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
34 kDa
Activity
Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °C for 20 minutes prior to gel electrophoresis.
Scientific Data Images for AQUApure Tetra-Ub Chains (K11-linked) Protein, CF
AQUApure Tetra-Ub Chains (K11-linked) Analysis
The correctness of linkage and purity of AQUApure Tetra-Ub Chains (K11-linked) Protein (Catalog #UC-45) was assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of Poly-Ubiquitin samples.Formulation, Preparation and Storage
UC-45
| Formulation | Supplied as a solution in deionized water. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Tetra-Ubiquitin
Ubiquitin chains vary in length, linkage, and function. K11-linked Tetra-Ubiquitin Chains (Ub4) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Linkage specific Poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB's") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF's and/or other Ubiquitin-sensing elements. K11-linked Tetra-Ubiquitin chains are manufactured using recombinant Ubiquitin and purely enzymatic techniques to avoid the potential for contaminating synthetic intermediates.
References
- Kirkpatrick, D.S., et al. (2006) Nat Cell Biol. 8:700.
- Ordureau, A., et al. (2014) Mol. Cell 56:360.
- Ordureau, A., et al. (2015) Pro. Nat. Acad. of Sci. USA 112: 6637.
- Phu, L., et al. (2011) Mol Cell Proteomics 10: M110.003756.
Alternate Names
TetraUbiquitin, Ub4
Entrez Gene IDs
7314 (Human)
Gene Symbol
UBB
UniProt
Additional Tetra-Ubiquitin Products
Product Documents for AQUApure Tetra-Ub Chains (K11-linked) Protein, CF
Product Specific Notices for AQUApure Tetra-Ub Chains (K11-linked) Protein, CF
For research use only
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