Recombinant Human Argininosuccinate Lyase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72510

Novus Biologicals, part of Bio-Techne
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NBP1-72510-0.5mg
NBP1-72510-100ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Argininosuccinate Lyase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-464 of Human Argininosuccinate Lyase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASESGKLWG GRFVGAVDPI MEKFNASIAY DRHLWEVDVQ GSKAYSRGLE KAGLLTKAEM DQILHGLDKV AEEWAQGTFK LNSNDEDIHT ANERRLKELI GATAGKLHTG RSRNDQVVTD LRLWMRQTCS TLSGLLWELI RTMVDRAEAE RDVLFPGYTH LQRAQPIRWS HWILSHAVAL TRDSERLLEV RKRINVLPLG SGAIAGNPLG VDRELLRAEL NFGAITLNSM DATSERDFVA EFLFWASLCM THLSRMAEDL ILYCTKEFSF VQLSDAYSTG SSLMPQKKNP DSLELIRSKA GRVFGRCAGL LMTLKGLPST YNKDLQEDKE AVFEVSDTMS AVLQVATGVI STLQIHQENM GQALSPDMLA TDLAYYLVRK GMPFRQAHEA SGKAVFMAET KGVALNQLSL QELQTISPLF SGDVICVWDY GHSVEQYGAL GGTARSSVDW QIRQVRALLQ AQQA

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

53.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Argininosuccinate Lyase His Protein

SDS-PAGE: Recombinant Human Argininosuccinate Lyase His Protein [NBP1-72510]

SDS-PAGE: Recombinant Human Argininosuccinate Lyase His Protein [NBP1-72510]

SDS-Page: Recombinant Human Argininosuccinate Lyase Protein [NBP1-72510] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72510
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 10% glycerol, 100 mM NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Argininosuccinate Lyase

ASL (argininosuccinate lyase) belongs to the lyase 1 family. ASL is an enzyme that catalyzes the reversible breakdown of argininosuccinate (ASA) producing the amino acids arginine and fumarate. Located in liver cytosol, ASL is the fourth enzyme of the urea cycle and involved in the biosynthesis of arginine in all species and the production of urea in ureotelic species. While ASS (argininosuccinate synthetase) catalyzes the formation of argininosuccinate from citrulline and aspartate, ASL breaks the newly formed argininosuccinate into L-arginine and fumarate. L-arginine continues through the urea cycle to form urea and orinthine, while fumarate can enter the citric acid cycle. Recombinant human ASL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

argininosuccinase, argininosuccinate lyase, Arginosuccinase, ASAL, EC 4.3.2.1

Gene Symbol

ASL

Additional Argininosuccinate Lyase Products

Product Documents for Recombinant Human Argininosuccinate Lyase His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Argininosuccinate Lyase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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