Recombinant Human ASF1a His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-22941

Novus Biologicals, part of Bio-Techne
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NBC1-22941-0.1mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all ASF1a Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-204 of Human ASF1a

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAKV QVNNVVVLDN PSPFYNPFQF EITFECIEDL SEDLEWKIIY VGSAESEEYD QVLDSVLVGP VPAGRHMFVF QADAPNPGLI PDADAVGVTV VLITCTYRGQ EFIRVGYYVN NEYTETELRE NPPVKPDFSK LQRNILASNP RVTRFHINWE DNTEKLEDAE SSNPNLQSLL STDALPSASK GWSTSENSLN VMLESHMDCM

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ASF1a His Protein

SDS-PAGE: Recombinant Human ASF1a His Protein [NBC1-22941]

SDS-PAGE: Recombinant Human ASF1a His Protein [NBC1-22941]

SDS-Page: Recombinant Human ASF1a His Protein [NBC1-22941] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBC1-22941
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
Preservative No Preservative
Concentration 1.0 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ASF1a

Histone chaperone ASF1A, CCG1-interacting factor A (CIA), CGI-98, HSPC146 Description ASF1 anti-silencing function 1 homolog A, also known as ASF1A, belongs to H3/H4 family of histone chaperone proteins. ASF1A protein interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. Deletion of ASF1A in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint. Recombinant human ASF1A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

ASF1 silencing function 1 homolog A (S. cerevisiae), CCG1-interacting factor A, CIACGI-98, DKFZP547E2110, hAsf1, hAsf1a, hCIA, histone chaperone ASF1A, HSPC146, silencing function 1A, silencing function protein 1 homolog A

Gene Symbol

ASF1A

Additional ASF1a Products

Product Documents for Recombinant Human ASF1a His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human ASF1a His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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