What kind of beta-lactamase does this antibody belong to? A, B, C or D? And what's molecular weight of beta-lactamase? Is it in already in solution or just solid?

Name: Recombinant E. coli beta-lactamase His Protein
Brand: Novus Biologicals
SKU: NBP1-72284

This antibody recognizes Beta-Lactamase, class C. The molecular weight is 41.8 kDa (379aa) confirmed by MALDI-TOF. The sequence falls between 20-377aa of UniProt P00811 (https://www.uniprot.org/uniprotkb/P00811/entry). Protein accession number: NP_418574. This product is sold in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol at 1.0mg/ml.

Recombinant E. coli beta-lactamase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72284

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all beta-lactamase Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 20-377 of E.coli Beta Lactamase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

41.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli beta-lactamase His Protein

SDS-PAGE: Recombinant E. coli beta-lactamase His Protein [NBP1-72284]

SDS-Page: Recombinant E. coli Beta Lactamase Protein [NBP1-72284] - 15% SDS-PAGE (3 ug)

Formulation, Preparation and Storage

NBP1-72284

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta-lactamase

AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E.coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

ampA, AmpC, cephalosporinase, EC 3.5.2.6, ECK4146

Gene Symbol

ampC

Additional beta-lactamase Products

Product Documents for Recombinant E. coli beta-lactamase His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant E. coli beta-lactamase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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