Recombinant E. coli beta-lactamase His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59641
Key Product Details
Source
E. coli
Tag
His
Conjugate
Unconjugated
Applications
Enzyme Activity, SDS-PAGE
Product Specifications
Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 20-377 of E. coli Beta Lactamase
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ
Purity
>95%, by SDS-PAGE
Predicted Molecular Mass
41.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Specific activity is >700 units/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant E. coli beta-lactamase His Protein
![SDS-PAGE: Recombinant E. coli beta-lactamase His Protein [NBP2-59641]](https://resources.bio-techne.com/images/products/Recombinant-E.-coli-Beta-Lactamase-Protein-SDS-Page-NBP2-59641-img0001.jpg "SDS-PAGE: Recombinant E. coli beta-lactamase His Protein [NBP2-59641]")
SDS-PAGE: Recombinant E. coli beta-lactamase His Protein [NBP2-59641]
SDS-Page: Recombinant E. coli Beta Lactamase Protein [NBP2-59641] - 15% SDS-PAGE (3ug)Formulation, Preparation and Storage
NBP2-59641
| Formulation | 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: beta-lactamase
Alternate Names
ampA, AmpC, cephalosporinase, EC 3.5.2.6, ECK4146
Gene Symbol
ampC
Additional beta-lactamase Products
Product Documents for Recombinant E. coli beta-lactamase His Protein
Product Specific Notices for Recombinant E. coli beta-lactamase His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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