Recombinant Human Betacellulin/BTC Protein

Name: Recombinant Human Betacellulin/BTC Protein
Brand: Novus Biologicals
SKU: NBP2-34919

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34919

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P35070

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Betacellulin/BTC Proteins and Enzymes »

Product Specifications

Description

Recombinant human Betacellulin is a 9.0 kDa monomeric protein, corresponding to 80 amino residues, which comprises the mature EGF homologous portion of Betacellulin/BTC Source: E. coli

Uniprot ID: P35070

Amino Acid Sequence: DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Betacellulin/BTC as determined by LAL method.

Predicted Molecular Mass

9.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Betacellulin/BTC Protein

SDS-PAGE: Recombinant Human Betacellulin/BTC Protein [NBP2-34919]

SDS-Page: Human Betacellulin/BTC Protein [NBP2-34919]

Formulation, Preparation and Storage

NBP2-34919

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Betacellulin/BTC

Betacellulin (BTC) encoded by the BTC gene located on the chromosome 4, is a member of the EGF family that also includes EGF, TGF-alpha, Amphiregulin, HB-EGF, Epiregulin, Tomoregulin and the Neuregulins. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids, including serum, milk, and colostrum. Mouse BTC is expressed as a 178-amino acid precursor and the amino acid sequence of the mature form is 80 % identical with human BTC. Both human and mouse BTC exhibit significant overall similarity with other members of the EGF family. Recombinant murine Betacellulin is a heparin binding protein containing 80 amino acids residues.

Alternate Names

BTC

Gene Symbol

BTC

UniProt