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Recombinant Human Calcineurin B His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78797

Novus Biologicals, part of Bio-Techne
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NBP1-78797-0.5mg
NBP1-78797-100ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-170 of Human Calcineurin B

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

21.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Calcineurin B His Protein

SDS-PAGE: Recombinant Human Calcineurin B His Protein [NBP1-78797]

SDS-PAGE: Recombinant Human Calcineurin B His Protein [NBP1-78797]

SDS-Page: Recombinant Human Calcineurin B Protein [NBP1-78797] - 15% SDS-PAGE (3 ug).

Formulation, Preparation and Storage

NBP1-78797
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT, 0.1 M NaCl
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calcineurin B

PPP3R1, also known as Calcineurin subunit B type 1, is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase that plays an essential role in the T cell activation pathway. Calcineurin is composed of two subunits; calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. Recombinant human PPP3R1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CALNB1, Cnb1, MCIP1, PP2B beta 1, PPP3R1

Gene Symbol

PPP3R1

Additional Calcineurin B Products

Product Documents for Recombinant Human Calcineurin B His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Calcineurin B His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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