Recombinant Mouse CCL21/6Ckine Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35179
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity, SDS-PAGE
Product Specifications
Description
A single, non-glycosylated polypeptide chain containing 110 amino acids corresponding to CCL21/6Ckine Source: E. coli
Uniprot ID: P86792
Amino Acid Sequence: SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of CCL21/6Ckine as determined by LAL method.
Predicted Molecular Mass
12.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
CCL21 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine T-lymphocytes is in a concentration range of 10-100 ng/ml.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Mouse CCL21/6Ckine Protein
![SDS-PAGE: Recombinant Mouse CCL21/6Ckine Protein [NBP2-35179]](https://resources.bio-techne.com/images/products/Mouse-CCL21-6Ckine-Protein-SDS-Page-NBP2-35179-img0001.jpg "SDS-PAGE: Recombinant Mouse CCL21/6Ckine Protein [NBP2-35179]")
SDS-PAGE: Recombinant Mouse CCL21/6Ckine Protein [NBP2-35179]
SDS-Page: Mouse CCL21/6Ckine Protein [NBP2-35179]Formulation, Preparation and Storage
NBP2-35179
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: CCL21/6Ckine
Alternate Names
6Ckine, exodus-2, SCYA21, SLC, TCA-4, Exodus-2/CCL21
Gene Symbol
CCL21
Additional CCL21/6Ckine Products
Product Documents for Recombinant Mouse CCL21/6Ckine Protein
Product Specific Notices for Recombinant Mouse CCL21/6Ckine Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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