Recombinant Human CCL25/TECK Protein

Name: Recombinant Human CCL25/TECK Protein
Brand: Novus Biologicals
SKU: NBP2-35039

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35039

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

O15444

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL25/TECK Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 128 amino acids corresponding to CCL25/TECK Source: E. coli

Uniprot ID: O15444

Amino Acid Sequence: MQGVFEDCCL AYHYPIGWAV LRRAWTYRIQ EVSGSCNLPA AIFYLPKRHR KVCGNPKSRE VQRAMKLLDA RNKVFAKLHH NTQTFQAGPH AVKKLSSGNS KLSSSKFSNP ISSSKRNVSL LISANSGL

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL25/TECK as determined by LAL method.

Predicted Molecular Mass

14.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

TECK protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL25/TECK Protein

SDS-PAGE: Recombinant Human CCL25/TECK Protein [NBP2-35039]

SDS-Page: Human CCL25/TECK Protein [NBP2-35039]

Formulation, Preparation and Storage

NBP2-35039

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL25/TECK

CCL25 is new member of CC family chemokine. It is also called Thymus-expressed chemokine (TECK) because it is restricted produced by thymus and intestine. Especially, the dendritic cells derived from thymus but not bone marrow had identified to be the source of CCL25. By binding with CCR9, it elicits its effects of chemotactic for thymocytes, macrophages, and dendritic cells. Additionally, CCL25 takes part in regulating the development of T-cells.

Alternate Names

TECK, CCL25, TECK/CCL25

Gene Symbol

CCL25

UniProt