Recombinant Mouse CCL27/CTACK Protein

Name: Recombinant Mouse CCL27/CTACK Protein
Brand: Novus Biologicals
SKU: NBP2-35164

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35164

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL27/CTACK Proteins and Enzymes »

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 95 amino acids corresponding to CCL27/CTACK Source: E. coli

Uniprot ID: Q9Z1X0

Amino Acid Sequence: LPLPSSTSCC TQLYRQPLPS RLLRRIVHME LQEADGDCHL QAVVLHLARR SVCVHPQNRS LARWLERQGK RLQGTVPSLN LVLQKKMYSN PQQQN

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL27/CTACK as determined by LAL method.

Predicted Molecular Mass

10.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL27 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CCL27/CTACK Protein

SDS-PAGE: Recombinant Mouse CCL27/CTACK Protein [NBP2-35164]

SDS-Page: Mouse CCL27/CTACK Protein [NBP2-35164]

Formulation, Preparation and Storage

NBP2-35164

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4, with 5% trehalose.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL27/CTACK

CCL27, also named IL-11 R-alpha-locus chemokine (ILC), skinkine, eskine and cutaneous T-cell-attracting chemokine (CTACK), is a small cytokine belonging to the CC chemokine family. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin and it elicits its chemotactic effects by binding to the chemokine receptor CCR10. CCL27 can attract skin-associated memory T-lymphocytes. Studies showed it may play a role in mediating homing of lymphocytes to cutaneous sites and cell migration during embryogenesis. Mature mouse CCL27 is a 95 amino acid (a.a.) protein that shares 57 % a.a. and 87 % a.a. sequence identity with human and rat CCL27, respectively. It shares 18 %~31 % a.a. sequence identity with other mouse CC chemokines.

Alternate Names

CTACK, ILC, CTACK/CCL27

Gene Symbol

CCL27

Additional CCL27/CTACK Products

Product Documents for Recombinant Mouse CCL27/CTACK Protein

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COA

Product Specific Notices for Recombinant Mouse CCL27/CTACK Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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