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Recombinant Human CCL3L1/LD78 beta Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34932

Novus Biologicals, part of Bio-Techne
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NBP2-34932-100ug
NBP2-34932-1mg
NBP2-34932-500ug
NBP2-34932-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

Approximately 7.8 kDa protein corresponding to 70 amino acid residues of CCL3L1/LD78 beta, including the four highly conserved cysteine residues present in CC chemokines Source: E. coli

Uniprot ID: P16619

Amino Acid Sequence: APLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL3L1/LD78 beta as determined by LAL method.

Predicted Molecular Mass

7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL3L1 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-34932
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL3L1/LD78 beta

CCL3L1, also named LD78-beta, is belonging to the intercrine beta (chemokine CC) family and it is encoded by the CCL3L1 gene in humans. This protein binds to several chemokine receptors including chemokine binding protein 2 (CCBP2 or D6) and chemokine (C-C motif) receptor 5 (CCR5). It is an inhibitor of HIV-1-infection and chemotactic for lymphocytes and monocytes. Recombinant human LD78beta is a 7.8 kDa protein containing 70 amino acid residues, including the four conserved cysteine residues present in CC chemokines.

Alternate Names

C-C motif chemokine 3-like 1, CCL3L3, chemokine (C-C motif) ligand 3-like 1, D17S1718, G0/G1 switch regulatory protein 19-2, G0S19-2LD78, LD78BETA, LD78-beta(1-70), MGC104178, MGC12815, MGC182017, MIP1AP, PAT 464.2, SCYA3L, SCYA3L1464.2, small inducible cytokine A3-like 1, Small-inducible cytokine A3-like 1, Tonsillar lymphocyte LD78 beta protein

Gene Symbol

CCL3L1

UniProt

Additional CCL3L1/LD78 beta Products

Product Documents for Recombinant Human CCL3L1/LD78 beta Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human CCL3L1/LD78 beta Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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