Skip to main content

Recombinant Human CCL4L1/LAG-1 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34929

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP2-34929-100ug
NBP2-34929-1mg
NBP2-34929-500ug
NBP2-34929-5ug

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 69 amino acids corresponding to CCL4L1/LAG-1 Source: E. coli

Uniprot ID: Q8NHW4

Amino Acid Sequence: APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of CCL4L1/LAG-1 as determined by LAL method.

Predicted Molecular Mass

7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CCL4L1 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human CCR5 transfected murine BaF3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL4L1/LAG-1 Protein

SDS-PAGE: Recombinant Human CCL4L1/LAG-1 Protein [NBP2-34929]

SDS-PAGE: Recombinant Human CCL4L1/LAG-1 Protein [NBP2-34929]

SDS-Page: Human CCL4L1/LAG-1 Protein [NBP2-34929]

Formulation, Preparation and Storage

NBP2-34929
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS.
Preservative No Preservative
Concentration Lyoph
Reconstitution Centrifuge prior to opening. Reconstitute in sterile dH2O or aq buffer containing 0.1% BSA to a conc of 1mg/mL. Apportion stock solutions into working aliquots and store at <-20C. Make further dilutions in buffered solution with NaCl conc >300mM.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL4L1/LAG-1

C-C motif chemokine 4-like, also known as MIP-1-beta, is encoded by one of several cytokine genes clustered on the q-arm of chromosome 17 and is similar to CCL4 which inhibits HIV entry by binding to the cellular receptor CCR5. The human CCL4L1 cDNA encodes a 92 amino acid (a.a.) precursor with a 23 a.a. signal sequence and it shares greater than 98% a.a. sequence identity with CCL4 and CCL4L2. Recombinant Human CCL4L1 also shares 96% a.a. sequence identity with rhesus CCL4 and approximately 80 ~ 90% a.a. sequence identity with bovine, murine, rabbit, and rat CCL4.

Alternate Names

LAG-1, LAG1, SCYA4L, LAG-1/CCL4L1

Gene Symbol

CCL4L2

Additional CCL4L1/LAG-1 Products

Product Documents for Recombinant Human CCL4L1/LAG-1 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human CCL4L1/LAG-1 Protein

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...