Recombinant Human CCL8/MCP-2 Protein

Name: Recombinant Human CCL8/MCP-2 Protein
Brand: Novus Biologicals
SKU: NBP2-34969

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34969

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P80075

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CCL8/MCP-2 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 76 amino acids corresponding to CCL8/MCP-2 Source: E. coli

Uniprot ID: P80075

Amino Acid Sequence: QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CCL8/MCP-2 as determined by LAL method.

Predicted Molecular Mass

8.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CCL8/MCP-2 Protein

SDS-PAGE: Recombinant Human CCL8/MCP-2 Protein [NBP2-34969]

SDS-Page: Human CCL8/MCP-2 Protein [NBP2-34969]

Formulation, Preparation and Storage

NBP2-34969

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CCL8/MCP-2

Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1- beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells.

Alternate Names

MCP-2, MCP2, CCL8, MCP-2/CCL8

Gene Symbol

CCL8

UniProt