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Recombinant Human CD3 zeta His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61462

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-61462-100ug
NBP2-61462-20ug

Key Product Details

Source

Baculovirus

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a C-terminal His-tag and corresponding to the amino acids 52-164 of Human CD3.

Source: Baculovirus

Amino Acid Sequence: ADPRVKFSRS ADAPAYQQGQ NQLYNELNLG RREEYDVLDK RRGRDPEMGG KPQRRKNPQE GLYNELQKDK MAEAYSEIGM KGERRRGKGH DGLYQGLSTA TKDTYDALHM QALPPRHHHH HH

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1ug of protein (determined by LAL method)

Predicted Molecular Mass

14.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CD3 zeta His Protein

SDS-PAGE: Recombinant Human CD3 zeta His Protein [NBP2-61462]

SDS-PAGE: Recombinant Human CD3 zeta His Protein [NBP2-61462]

SDS-Page: Recombinant Human CD3 Protein [NBP2-61462] - 15% SDS-Page (3ug)

Formulation, Preparation and Storage

NBP2-61462
Formulation 20 mM Tris-HCl buffer (pH 6.8), 50% glycerol, 1 mM DTT, 1 mM EDTA, 0.1 M NaCl
Preservative No Preservative
Concentration 0.2 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 zeta/CD247

T-cell surface glycoprotein CD3 zeta chain, also known as CD247, belongs to the CD3Z/FCER1G family. CD247 is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Recombinant human CD247 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Gene Symbol

CD247

Additional CD3 zeta/CD247 Products

Product Documents for Recombinant Human CD3 zeta His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human CD3 zeta His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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