Recombinant Human CD68/SR-D1 His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53081
Key Product Details
Source
Baculovirus
Tag
His
Conjugate
Unconjugated
Applications
SDS-PAGE
Product Specifications
Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids22-319 of Human CD68/SR-D1
Source:Baculovirus
Amino Acid Sequence: ADPNDCPHKK SATLLPSFTV TPTVTESTGT TSHRTTKSHK TTTHRTTTTG TTSHGPTTAT HNPTTTSHGN VTVHPTSNST ATSQGPSTAT HSPATTSHGN ATVHPTSNST ATSPGFTSSA HPEPPPPSPS PSPTSKETIG DYTWTNGSQP CVHLQAQIQI RVMYTTQGGG EAWGISVLNP NKTKVQGSCE GAHPHLLLSF PYGHLSFGFM QDLQQKVVYL SYMAVEYNVS FPHAAQWTFS AQNASLRDLQ APLGQSFSCS NSSIILSPAV HLDLLSLRLQ AAQLPHTGVF GQSFSCPSDR SHHHHHH
Purity
>95%, by SDS-PAGE
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Predicted Molecular Mass
32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human CD68/SR-D1 His Protein
![SDS-PAGE: Recombinant Human CD68/SR-D1 His Protein [NBP2-53081]](https://resources.bio-techne.com/images/products/Recombinant-Human-CD68-SR-D1-Protein-SDS-Page-NBP2-53081-img0002.jpg "SDS-PAGE: Recombinant Human CD68/SR-D1 His Protein [NBP2-53081]")
SDS-PAGE: Recombinant Human CD68/SR-D1 His Protein [NBP2-53081]
SDS-Page: Recombinant Human CD68/SR-D1 Protein [NBP2-53081]Formulation, Preparation and Storage
NBP2-53081
| Formulation | PBS (pH 7.4), 10% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: CD68/SR-D1
CD68 is highly expressed in cells of the mononuclear phagocyte system such as macrophages, microglia, osteoclasts, and myeloid dendritic cells (DCs); and is expressed to a lesser extent in lymphoid cells (CD19+ B lymphocytes and CD4+ T lymphocytes), human umbilical cord mesenchymal stem cells (MSCs), fibroblasts, endothelial cells, multiple non-hematopoietic cancer cell lines, and human arterial intimal smooth muscle cells (SMCs). Expression has been also observed in diseased states for granulocytes and neutrophils, in particular basophils from myeloproliferative disorders and intestinal neutrophils from inflammatory bowel disease (IBD), respectively (1).
Although the function of CD68 has yet to be established, it has often been used as an immunohistochemistry (IHC) marker of inflammation and for granular cell tumors (GCTs). CD68+ tumor associated macrophages (TAMs) has been suggested to be a predictive marker for poor cancer prognosis, but a meta-analysis showed the presence of CD68 is not correlated with survival (2). In addition, a role in hepatic malaria infection has been reported based on the finding that peptide P39 binds CD68, considered a receptor for malaria sporozoite, and inhibits parasite entry into Kupffer cells. CD68 was deemed a member of the Scavenger receptor family due to its upregulation in macrophages following inflammatory stimuli, ability to bind modified LDL, phosphatidylserine, and apoptotic cells, as well as shuttling between the plasma membrane and endosomes. CD68 has been linked to atherogenesis based on binding and internalization of its ligand, oxLDL (1).
References
1. Chistiakov, DA, Killingsworth, MC, Myasoedova, VA. Orekhov AN, Bobryshev YV. (2017) CD68/macrosialin: not just a histochemical marker. Lab Invest. 97:4-13. PMID: 27869795
2. Troiano G, Caponio VCA, Adipietro I, Tepedino M, Santoro R, Laino L, Lo Russo L, Cirillo N, Lo Muzio L. (2019) Prognostic significance of CD68+ and CD163+ tumor associated macrophages in head and neck squamous cell carcinoma: A systematic review and meta-analysis. Oral Oncol. 93:66-75. PMID: 31109698.
Alternate Names
CD68, gp110, Macrosialin, SCARD1, SR-D1, SRD1
Gene Symbol
CD68
Additional CD68/SR-D1 Products
Product Documents for Recombinant Human CD68/SR-D1 His Protein
Product Specific Notices for Recombinant Human CD68/SR-D1 His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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