Recombinant Human CDK4 His Protein

Name: Recombinant Human CDK4 His Protein
Brand: Novus Biologicals
SKU: NBP2-22974

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22974

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

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Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-303 of Human CDK4

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMATSRYE PVAEIGVGAY GTVYKARDPH SGHFVALKSV RVPNGGGGGG GLPISTVREV ALLRRLEAFE HPNVVRLMDV CATSRTDREI KVTLVFEHVD QDLRTYLDKA PPPGLPAETI KDLMRQFLRG LDFLHANCIV HRDLKPENIL VTSGGTVKLA DFGLARIYSY QMALTPVVVT LWYRAPEVLL QSTYATPVDM WSVGCIFAEM FRRKPLFCGN SEADQLGKIF DLIGLPPEDD WPRDVSLPRG AFPPRGPRPV QSVVPEMEES GAQLLLEMLT FNPHKRISAF RALQHSYLHK DEGNPE

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

36.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CDK4 His Protein

SDS-PAGE: Recombinant Human CDK4 His Protein [NBP2-22974]

SDS-Page: Recombinant Human CDK4 Protein [NBP2-22974]

Formulation, Preparation and Storage

NBP2-22974

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDK4

Cyclin-dependent kinase 4, also known as CDK4, belongs to the Cyclin-dependent kinases (CDK) family. CDK are the catalytic subunits of the cyclin/cdk complexes, which phosphorylate substrates on threonine/serine residues. CDK4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates CDK4 activity, whereas binding to the CDK inhibitor p16 downregulates CDK4 activity. Recombinant human CDK4 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

Cyclin-dependent Kinase 4

Alternate Names

CMM3, PSK-J3

Gene Symbol

CDK4

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COA

Product Specific Notices for Recombinant Human CDK4 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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