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Recombinant Human CENPH His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72449

Novus Biologicals, part of Bio-Techne
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NBP1-72449-0.5mg
NBP1-72449-100ug

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 136-247 of Human CENPH

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

15.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CENPH His Protein

SDS-PAGE: Recombinant Human CENPH His Protein [NBP1-72449]

SDS-PAGE: Recombinant Human CENPH His Protein [NBP1-72449]

SDS-Page: Recombinant Human CENPH Protein [NBP1-72449] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72449
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CENPH

CENPH, also known as centromere protein H, belongs to the centromere protein H family. This protein is component of the CENPA-NAC (nucleosome-associated) complex that plays a central role in assembly of kinetochore proteins. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Recombinant human CENPH protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process.

Alternate Names

CENP-H, centromere protein H, ICEN35, Interphase centromere complex protein 35, kinetochore protein CENP-H, NNF1, PMF1

Gene Symbol

CENPH

Additional CENPH Products

Product Documents for Recombinant Human CENPH His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human CENPH His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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