CHMP4A Recombinant Protein Antigen

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49557PEP

Novus Biologicals, part of Bio-Techne
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NBP2-49557PEP
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Antibody Competition

View all CHMP4A Proteins and Enzymes »

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human CHMP4A.

Source: E. coli

Amino Acid Sequence: THLPAGPAPKVDEDEEALKQLAEWVS

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-49557.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation and Storage

NBP2-49557PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: CHMP4A

CHMP4A (charged MVB protein 4A) is one of the three isoforms of CHMP4, the human equivalent of yeast Snf7 (so CHMP4A is also referred to as hSnf7-1). It is a component of the ESCRT-III (endosomal sorting complex required for transport III) complex. The ESCRT complexes mediate membrane budding away from the cytosol and cleavage of membrane necks (membrane fission) from the cytosolic face; hence, they are key in processes such as the formation of MVBs (multivesicular bodies), enveloped virus budding, and membrane abscission in cytokinesis. Not all types of ESCRT complexes, or proteins within those complexes, are necessary for all of these functions. For instance, research into the requirements of ESCRT-III proteins in HIV-1 budding has revealed that CHMP1 and CHMP3 proteins are not essential for this process; at least one member of each of the CHMP2 and CHMP4 families are required; within these families there is some functional redundancy; within the CHMP4 family, the relative importance and abundance of the isoforms is 4B > 4A > 4C; and the absence of CHMP4A or 4B prevents the budding process from progressing to completion.

Alternate Names

C14orf123, charged multivesicular body protein 4a, CHMP4, CHMP4a, CHMP4B, chromatin modifying protein 4A, Chromatin-modifying protein 4a, chromosome 14 open reading frame 123, FLJ61658, hSnf-1, HSPC134, hVps32-1, MGC142093, MGC142095, SHAX2, SNF7, SNF7 homolog associated with Alix-2, Snf7 homologue associated with Alix 2, SNF7-1, Vacuolar protein sorting-associated protein 32-1, Vps32-1, VPS32A

Gene Symbol

CHMP4A

Additional CHMP4A Products

Product Documents for CHMP4A Recombinant Protein Antigen

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CHMP4A Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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