Recombinant Human cleavage stimulation factor His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98871

Novus Biologicals, part of Bio-Techne
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NBP1-98871-0.5mg
NBP1-98871-100ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all cleavage stimulation factor Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-431 of Human cleavage stimulation factor

Source: E.coli

Amino Acid Sequence: MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DVEHHHHHH

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

49.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human cleavage stimulation factor His Protein

SDS-PAGE: Recombinant Human cleavage stimulation factor His Protein [NBP1-98871]

SDS-PAGE: Recombinant Human cleavage stimulation factor His Protein [NBP1-98871]

SDS-Page: cleavage stimulation factor Protein [NBP1-98871] - Analysis at 15% (3ug)

Formulation, Preparation and Storage

NBP1-98871
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 2M UREA
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: cleavage stimulation factor

CSTF1, also known as cleavage stimulation factor subunit 1, is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. Recombinant human CSTF1 protein, fused to His-tag at C-terminus, was expressed in E.coli.

Alternate Names

CF-1 50 kDa subunit, Cleavage stimulation factor 50 kDa subunit, cleavage stimulation factor subunit 1, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kD, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa, CSTF 50 kDa subunit, CstF-50, CstFp50

Gene Symbol

CSTF1

Additional cleavage stimulation factor Products

Product Documents for Recombinant Human cleavage stimulation factor His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human cleavage stimulation factor His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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