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Name: Recombinant Human Complexin-1 His Protein
Brand: Novus Biologicals
SKU: NBC1-18349

NBC1-18349: full length protein, aa 1-134, with his-tag.

Recombinant Human Complexin-1 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-18349

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Complexin-1 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-134 of Human Complexin-1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA KYAKMEAERE AVRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA IPPGCGDEVE EEDESILDTV IKYLPGPLQD MLKK

Purity

>90%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

17.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Complexin-1 His Protein

SDS-PAGE: Recombinant Human Complexin-1 His Protein [NBC1-18349]

SDS-Page: Recombinant Human Complexin-1 Protein [NBC1-18349] - Complexin 1, 17.1 kDa (154 aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-18349

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Complexin-1

Complexin1 belongs to a family of SNARE complex binding proteins that were suggested to be either facilitators or inhibitors of vesicle exocytosis. Complexin 1 exhibits reduced Ca2+-triggered fast neurotransmitter release at hippocampal glutamatergic synapses, indicating that this protein is a positive regulator of transmitter release. In contrast, it inhibits SNARE-mediated liposome and cell fusions in vitro, which lead to the hypothesis that it acts as fusion clamps of synaptic exocytosis. Recombinant Complexin 1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

CPLX1, Synaphin-2

Gene Symbol

CPLX1

Additional Complexin-1 Products

Product Documents for Recombinant Human Complexin-1 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Complexin-1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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