Recombinant Human Creatine kinase MT 1B His Protein

Name: Recombinant Human Creatine kinase MT 1B His Protein
Brand: Novus Biologicals
SKU: NBP1-98939

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-98939

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Enzyme Activity, SDS-PAGE

View all Creatine kinase MT 1B Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 40-417 of Human Creatine kinase MT 1B

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASERR RLYPPSAEYP DLRKHNNCMA SHLTPAVYAR LCDKTTPTGW TLDQCIQTGV DNPGHPFIKT VGMVAGDEET YEVFADLFDP VIQERHNGYD PRTMKHTTDL DASKIRSGYF DERYVLSSRV RTGRSIRGLS LPPACTRAER REVERVVVDA LSGLKGDLAG RYYRLSEMTE AEQQQLIDDH FLFDKPVSPL LTAAGMARDW PDARGIWHNN EKSFLIWVNE EDHTRVISME KGGNMKRVFE RFCRGLKEVE RLIQERGWEF MWNERLGYIL TCPSNLGTGL RAGVHIKLPL LSKDSRFPKI LENLRLQKRG TGGVDTAATG GVFDISNLDR LGKSEVELVQ LVIDGVNYLI DCERRLERGQ DIRIPTPVIH TKH

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 50 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of phosphate from phosphocreatine to ADP per minute at pH 7.5 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Creatine kinase MT 1B His Protein

SDS-PAGE: Recombinant Human Creatine kinase MT 1B His Protein [NBP1-98939]

SDS-Page: Creatine kinase MT 1B Protein [NBP1-98939] - 15% SDS-PAGE (3 ug).

Formulation, Preparation and Storage

NBP1-98939

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Creatine kinase MT 1B

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Recombinant human CKMT1A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Acidic-Type Mitochondrial Creatine Kinase, CKMT, CKMT1, CKMT1B, Creatine Kinase U-Type, Mitochondrial, Creatine Kinase, Mitochondrial 1 (Ubiquitous), Creatine Kinase, Mitochondrial 1B, EC 2.7.3.2, Mia-CK, Ubiquitous Mitochondrial Creatine Kinase, UMTCK, U-MtCK

Gene Symbol

CKMT1B

Additional Creatine kinase MT 1B Products

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COA

Product Specific Notices for Recombinant Human Creatine kinase MT 1B His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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