Recombinant Human CrkL His Protein

Name: Recombinant Human CrkL His Protein
Brand: Novus Biologicals
SKU: NBP1-50941

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50941

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all CrkL Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-303 of Human CrkL

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSARFDSSD RSAWYMGPVS RQEAQTRLQG QRHGMFLVRD SSTCPGDYVL SVSENSRVSH YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE PAPRYPSPPM GSVSAPNLPT AEDNLEYVRT LYDFPGNDAE DLPFKKGEIL VIIEKPEEQW WSARNKDGRV GMIPVPYVEK LVRSSPHGKH GNRNSNSYGI PEPAHAYAQP QTTTPLPAVS GSPGAAITPL PSTQNGPVFA KAIQKRVPCA YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD ENE

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

35.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CrkL His Protein

SDS-PAGE: Recombinant Human CrkL His Protein [NBP1-50941]

SDS-Page: Recombinant Human CrkL Protein [NBP1-50941] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-50941

Formulation	20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT, 0.1 M NaCl
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CrkL

CRKL is a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substitutete of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and has oncogenic potential. Recombinant human CRKL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

V-Crk Sarcoma Virus CT10 Oncogene Homolog-like Protein

Alternate Names

crk-like protein, pCRKL, v-crk avian sarcoma virus CT10 oncogene homolog-like, v-crk sarcoma virus CT10 oncogene homolog (avian)-like

Gene Symbol

CRKL

Additional CrkL Products

Product Documents for Recombinant Human CrkL His Protein

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COA

Product Specific Notices for Recombinant Human CrkL His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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