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Recombinant Human CXCL1/GRO alpha/KC/CINC-1 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34904

Novus Biologicals, part of Bio-Techne
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NBP2-34904-100ug
NBP2-34904-1mg
NBP2-34904-500ug
NBP2-34904-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 73 amino acids corresponding to CXCL1/GRO alpha/KC/CINC-1 Source: E. coli

Uniprot ID: P09341

Amino Acid Sequence: ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL1/GRO alpha/KC/CINC-1 as determined by LAL method.

Predicted Molecular Mass

7.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

GRO alpha Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 10-50 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human CXCL1/GRO alpha/KC/CINC-1 Protein

SDS-PAGE: Recombinant Human CXCL1/GRO alpha/KC/CINC-1 Protein [NBP2-34904]

SDS-PAGE: Recombinant Human CXCL1/GRO alpha/KC/CINC-1 Protein [NBP2-34904]

SDS-Page: Human CXCL1 Protein [NBP2-34904]

Formulation, Preparation and Storage

NBP2-34904
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1 is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. The gene for CXCL1 was initially discovered in hamster cells. In addition to the GRO gene, two GRO genes, GRO- beta and GRO-gamma share 90% and 86% amino acid sequence identity with CXCL1/GRO-alpha. All three human GROs are members of the intercrine alpha (chemokine C-X-C) subfamily of chemokine. CXCL1 is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha, GRO-alpha, GRO-alpha/CXCL1

Gene Symbol

CXCL1

UniProt

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for Recombinant Human CXCL1/GRO alpha/KC/CINC-1 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human CXCL1/GRO alpha/KC/CINC-1 Protein

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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