Recombinant Mouse CXCL14/BRAK Protein

Name: Recombinant Mouse CXCL14/BRAK Protein
Brand: Novus Biologicals
SKU: NBP2-35156

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35156

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL14/BRAK Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 77amino acids corresponding to CXCL14/BRAK Source: E. coli

Uniprot ID: Q9WUQ5

Amino Acid Sequence: SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIVTTKS MSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL14/BRAK as determined by LAL method.

Predicted Molecular Mass

9.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL14 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35156

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 400 mM NaCl, pH 7.4.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL14/BRAK

Chemokine (C-X-C motif) ligand 14 (CXCL14), also named BRAK, is a small cytokine belonging to the CXC chemokine family. It is constitutively expressed at the mRNA level in certain normal tissues and it possesses chemoattractive activity for activated macrophages, immature dendritic cells and natural killer cells. According to the relevant literature, CXCL14 is associated with tumor development. Moreover, recent evidence revealed that CXCL14 participates in glucose metabolism, feeding behaviourassociated neuronal circuits, and anti-microbial defense. Although CXCL14 receptors have not yet been identified, the intracellular activity of CXCL14 in breast cancer cells suggests that the CXCL14 receptor(s) and signal transduction pathway(s) may be different from those of conventional CXC-type chemokines. Recombinant murine CXCL14 contains 77 amino acid residues and it shares 97 % and 99 % a.a. sequence identity with human and rat CXCL14.

Alternate Names

Bolekine, BRAK, CXC-X3, BRAK/CXCL14

Gene Symbol

CXCL14

Additional CXCL14/BRAK Products

Product Documents for Recombinant Mouse CXCL14/BRAK Protein

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COA

Product Specific Notices for Recombinant Mouse CXCL14/BRAK Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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