Recombinant Human CXCL2/GRO beta/MIP-2/CINC-3 Protein

Name: Recombinant Human CXCL2/GRO beta/MIP-2/CINC-3 Protein
Brand: Novus Biologicals
SKU: NBP2-34918

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34918

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Product Datasheet / COA

Catalog #

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P19875

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all CXCL2/GRO beta/MIP-2/CINC-3 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 73 amino acids corresponding to CXCL2/GRO beta/MIP-2/CINC-3 Source: E. coli

Uniprot ID: P19875

Amino Acid Sequence: APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSN

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL2/GRO beta/MIP-2/CINC-3 as determined by LAL method.

Predicted Molecular Mass

7.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL2 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-34918

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL2/GRO beta/MIP-2/CINC-3

CXCL2 is a small cytokine belonging to the CXC chemokine family that is also known as Chemokine (C-X-C motif) ligand 2, and macrophage inflammatory protein 2-alpha (MIP2-alpha). CXCL2 is secreted by activated monocytes, neutrophils and macrophages and expressed at sites of inflammation. CXCL2 is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells and suppresses hematopoietic progenitor cell proliferation. Recombinant human CXCL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2, CINC-3/CXCL2, Macrophage Inflammatory Protein, Macrophage Inflammatory Protein 2, MIP-2/CXCL2

Gene Symbol

CXCL2

UniProt