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Recombinant Mouse CXCL5/ENA-78 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35157

Novus Biologicals, part of Bio-Techne
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NBP2-35157-100ug
NBP2-35157-1mg
NBP2-35157-500ug
NBP2-35157-5ug

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

Product Specifications

Description

A single, non-glycosylated polypeptide chain containing 92 amino acids corresponding to CXCL5/ENA-78 Source: E. coli

Uniprot ID: P50228

Amino Acid Sequence: APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of CXCL5/ENA-78 as determined by LAL method.

Predicted Molecular Mass

9.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

CXCL5 is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse CXCL5/ENA-78 Protein

SDS-PAGE: Recombinant Mouse CXCL5/ENA-78 Protein [NBP2-35157]

SDS-PAGE: Recombinant Mouse CXCL5/ENA-78 Protein [NBP2-35157]

SDS-Page: Mouse CXCL5 Protein [NBP2-35157]

Formulation, Preparation and Storage

NBP2-35157
Formulation Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: CXCL5/ENA-78

CXCL5 is a small cytokine belonging to the CXC chemokine family and it is encoded by the CXCL5 gene. It May participate in the recruitment of inflammatory cells by injured or infected tissue. CXCL5 is cleaved into the following 2 chains: GCP-2 (1-78) and GCP-2 (9-78) which attract neutrophils and are involved in neutrophil activation. Recombinant Mouse CXCL5 contains 92 amino acids and shares 57 % amino acid sequence identity with human CXCL5.

Alternate Names

CXCL5, ENA-78, ENA78, LIX/CXCL5

Gene Symbol

CXCL5

UniProt

Additional CXCL5/ENA-78 Products

Product Documents for Recombinant Mouse CXCL5/ENA-78 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Mouse CXCL5/ENA-78 Protein

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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