Recombinant Human Cytochrome P450 2E1 His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-51999
Key Product Details
Source
E. coli
Tag
His
Conjugate
Unconjugated
Applications
SDS-PAGE
Product Specifications
Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 29-493 of Human Cytochrome P450 2E1
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQ RMVVMHGYKA VKEALLDYKD EFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNES RIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEK EKHSAERLYT MDGITVTVAD LFFAGTETTS TTLRYGLLIL MKYPEIEEKL HEEIDRVIGP SRIPAIKDRQ EMPYMDAVVH EIQRFITLVP SNLPHEATRD TIFRGYLIPK GTVVVPTLDS VLYDNQEFPD PEKFKPEHFL NENGKFKYSD YFKPFSTGKR VCAGEGLARM ELFLLLCAIL QHFNLKPLVD PKDIDLSPIH IGFGCIPPRY KLCVIPRS
Purity
>80%, by SDS-PAGE
Predicted Molecular Mass
56.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human Cytochrome P450 2E1 His Protein
![SDS-PAGE: Recombinant Human Cytochrome P450 2E1 His Protein [NBP2-51999]](https://resources.bio-techne.com/images/products/Cytochrome-P450-2E1-Recombinant-Protein-SDS-Page-NBP2-51999-img0001.jpg "SDS-PAGE: Recombinant Human Cytochrome P450 2E1 His Protein [NBP2-51999]")
SDS-PAGE: Recombinant Human Cytochrome P450 2E1 His Protein [NBP2-51999]
SDS-Page: Cytochrome P450 2E1 Recombinant Protein [NBP2-51999] - 15% SDS Page (3 ug)Formulation, Preparation and Storage
NBP2-51999
| Formulation | 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol |
| Preservative | No Preservative |
| Concentration | 0.5 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: Cytochrome P450 2E1
Alternate Names
4-nitrophenol 2-hydroxylase, CPE1, CYP2Ecytochrome P450 2E1, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, Cytochrome P450-J, EC 1.14.13.-, EC 1.14.13.n7, EC 1.14.14.1, flavoprotein-linked monooxygenase, microsomal monooxygenase, P450C2E, P450-J, xenobiotic monooxygenase
Gene Symbol
CYP2E1
Additional Cytochrome P450 2E1 Products
Product Documents for Recombinant Human Cytochrome P450 2E1 His Protein
Product Specific Notices for Recombinant Human Cytochrome P450 2E1 His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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