Recombinant Human Cytokeratin 16 GST (N-Term) Protein

Name: Recombinant Human Cytokeratin 16 GST (N-Term) Protein
Brand: Novus Biologicals
SKU: H00003868-P01
Rating: 5 (1 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # H00003868-P01

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Citations (1)

Product Datasheet / COA / SDS

Discontinued Product

H00003868-P01 has been discontinued. View all Cytokeratin 16 products.

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

Wheat germ

Tag

GST (N-Term)

Conjugate

Unconjugated

Applications

Dot Blot, ELISA, Affinity Purification, Microarray, Western Blot

View all Cytokeratin 16 Proteins and Enzymes »

Product Specifications

Description

Recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-473 of Human KRT16

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSRFSSGGACGLGGGYGGGFSSSSSFGSGFGGGYGGGLGAGFGGGLGAGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPSEIKDYSPYFKTIEDLRNKIIAATIENAQPILQIDNARLAADDFRTKYEHELALRQTVEADVNGLRRVLDELTLARTDLEMQIEGLKEELAYLRKNHEEEMLALRGQTGGDVNVEMDAAPGVDLSRILNEMRDQYEQMAEKNRRDAETWFLSKTEELNKEVASNSELVQSSRSEVTELRRVLQGLEIELQSQLSMKASLENSLEETKGRYCMQLSQIQGLIGSVEEQLAQLRCEMEQQSQEYQILLDVKTRLEQEIATYRRLLEGEDAHLSSQQASGQSYSSREVFTSSSSSSSRQTRPILKEQSSSSFSQGQSS

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

77.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.

Application Notes

Use in Dot Blot reported in scientific literature (PMID 27773721).

Protein / Peptide Type

Recombinant Protein

Reviewed Applications

Read 1 review rated 5 using H00003868-P01 in the following applications:

Binding assay/Protein-protein interaction (1 Review)

Scientific Data Images for Recombinant Human Cytokeratin 16 GST (N-Term) Protein

SDS-PAGE: Recombinant Human Cytokeratin 16 GST (N-Term) Protein [H00003868-P01]

SDS-Page: Recombinant Human Cytokeratin 16 Protein [H00003868-P01]

Formulation, Preparation and Storage

H00003868-P01

Preparation Method	in vitro wheat germ expression system
Formulation	50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative	No Preservative
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 16

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq]

Alternate Names

CK16, CK-16, cytokeratin 16, Cytokeratin-16, FNEPPK, focal non-epidermolytic palmoplantar keratoderma, K16, K1CP, keratin 16, keratin, type I cytoskeletal 16, keratin-16, KRT16A, NEPPK

Gene Symbol

KRT16

Additional Cytokeratin 16 Products

Product Documents for Recombinant Human Cytokeratin 16 GST (N-Term) Protein

Product Datasheets

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COA

SDS

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Product Specific Notices for Recombinant Human Cytokeratin 16 GST (N-Term) Protein

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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