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DHX32 Recombinant Protein Antigen

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13921PEP

Novus Biologicals, part of Bio-Techne
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NBP2-13921PEP

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Antibody Competition

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human DHX32.

Source: E. coli

Amino Acid Sequence: EKGDIVVFLACEQDIEKVCETVYQGSNLNPDLGELVVVPLYPKEKCSLFKPLDETEKRCQVYQRRVVLTTSSGEFLIWSNSVRFVIDVGVERR

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-13921.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation and Storage

NBP2-13921PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: DHX32

DHX32, also known as Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32, consists of an 84.4 kDa and a 75.1 kDa isoform and is used in splicing and unwinding RNA in lymphoid tissue and other muscular tissues. The protein is being studied for its involvement in leukemia, brain cancer, and colorectal cancer. As a part of the mRNA processing and ATP binding pathways, the protein interacts with UBC.

Alternate Names

DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 32, DEAD/H box 32, DEAD/H helicase-like protein 1, DEAH (Asp-Glu-Ala-His) box polypeptide 32, DEAH box protein 32, DHLP1DDX32DEAD/H helicase-like protein-1, EC 3.6.1, EC 3.6.4.13, FLJ10694, FLJ10889, huDDX32, putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

Gene Symbol

DHX32

Additional DHX32 Products

Product Documents for DHX32 Recombinant Protein Antigen

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DHX32 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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