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DLST Recombinant Protein Antigen

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85012PEP

Novus Biologicals, part of Bio-Techne
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NBP1-85012PEP

Key Product Details

Source

E. coli

Tag

N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

Conjugate

Unconjugated

Applications

Antibody Competition

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human DLST.

Source: E. coli

Amino Acid Sequence: QNTCAMLTTFNEIDMSNIQEMRARHKEAFLKKHNLKLGFMSAFVKASAFALQEQPVVNAVIDDTTKEVVYRDYIDISVAVATPRGLVVPVIRNVEAMNFADIERTITELGEKARKNELAIEDMDGGTFTISNGGVFGSLFGTPII

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-85012.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation and Storage

NBP1-85012PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: DLST

DLST, also known as Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, is a 48.8 kDa, 453 amino acid protein that is involved in catalyzing the reaction of 2-oxoglutarate into succinyl-CoA and carbon dioxide. Current research is investigating the effect of the protein on malaria, neurodegeneration, pneumonia, cat-scratch disease, ataxia, prostatitis, Parkinson's disease, and Alzheimer's disease. The protein is involved in lysine degradation, citric acid cycle (TCA cycle), and metabolism pathways where it interacts with FLII, ATP6V1B2, HSPD1, UMPS, and DDOST.

Alternate Names

2-oxoglutarate dehydrogenase complex component E2, dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutaratecomplex), Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenasecomplex, dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutaratedehydrogenase complex, mitochondrial, DLTS, E2K, EC 2.3.1, EC 2.3.1.61, OGDC-E2

Gene Symbol

DLST

Additional DLST Products

Product Documents for DLST Recombinant Protein Antigen

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DLST Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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