DNASE1 Native Protein
Novus Biologicals, part of Bio-Techne | Catalog # P5224
Key Product Details
Source
Bovine pancreas
Conjugate
Unconjugated
Applications
Bioactivity, Enzyme Activity
Product Specifications
Description
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position.
Source: Native protein purified from Bovine Pancreas
Activity: >=2,000 Kunitz units per mg dry weight. One Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0
Source: Native protein purified from Bovine Pancreas
Activity: >=2,000 Kunitz units per mg dry weight. One Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0
Purity
>80% by SDS-PAGE and Coomassie blue staining
Predicted Molecular Mass
31.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Protein / Peptide Type
Native Protein
Formulation, Preparation and Storage
P5224
| Preparation Method | Native protein purified from Bovine Pancreas |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Reconstitute in many buffers pH 4.5-9.0. The choice of buffer is usually application specific. For cell culture applications some labs do reconstitute DNAse I in a neutral balanced salt solution(ie HANKS, EARLES, PBS) 0.22 um filter. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C. Do not freeze. |
Background: DNASE1
Alternate Names
deoxyribonuclease IFLJ38093, deoxyribonuclease-1, DKFZp686H0155, DNase I, DNase I, lysosomal, DNL1FLJ44902, Dornase alfa, DRNI, EC 3.1.21.1, human urine deoxyribonuclease I
Gene Symbol
DNASE1
Additional DNASE1 Products
Product Specific Notices for DNASE1 Native Protein
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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