Recombinant E. coli DsbE Protein

Name: Recombinant E. coli DsbE Protein
Brand: Novus Biologicals
SKU: NBC1-21275

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-21275

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all DsbE Proteins and Enzymes »

Product Specifications

Description

An un-tagged recombinant protein corresponding to the amino acids 26-185 of E.coli DsbE

Source: E.coli

Amino Acid Sequence: MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

18.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant E. coli DsbE Protein

SDS-PAGE: Recombinant E. coli DsbE Protein [NBC1-21275]

SDS-Page: Recombinant E. coli DsbE Protein [NBC1-21275] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBC1-21275

Formulation	20 mM Tris-HCl buffer (pH 7.5), 2 mM EDTA 10% glycerol
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DsbE

DsbE, also called CcmG, is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. Recombinant DsbE protein was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

ECK2187, yejQ

Gene Symbol

ccmG

Additional DsbE Products

Product Documents for Recombinant E. coli DsbE Protein

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COA

Product Specific Notices for Recombinant E. coli DsbE Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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