Recombinant Human EBI3 Protein

Name: Recombinant Human EBI3 Protein
Brand: Novus Biologicals
SKU: NBP2-76293

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76293

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

SDS-PAGE

View all EBI3 Proteins and Enzymes »

Product Specifications

Description

For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Purity

>90%, by SDS-PAGE

Endotoxin Level

Acceptance criteria for endotoxin levels are <5 EUs/ug protein (determined by Kinetic LAL Method).

Predicted Molecular Mass

23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Specificity

IL-27/IL-35 EBI3 Subunit is a monomer, 23.4 kDa (210 aa)

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EBI3 Protein

SDS-PAGE: Recombinant Human EBI3 Protein [NBP2-76293]

SDS-Page: Recombinant Human EBI3 Protein [NBP2-76293] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Formulation, Preparation and Storage

NBP2-76293

Preparation Method	Protein is derived from genetically modified E. coli.
Formulation	Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) + 0.5% mannitol
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Sterile 20 mM HCI at 0.1 mg/mL
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: EBI3

EBI3 gene encodes a 229 amino acid long, 25 kDA interleukin-27 subunit beta protein that is member to the hematopoitetin receptor family. These proteins obtain pro as well as anti-inflammatory properties and have roles in T-helper cell development, suppressing cell proliferation, encouraging cytotoxic T-cell activity, and can induce isotope switching in B cells. EBI3 participates in Th1 differentiation, IL-2 signaling (and its primary biological effects in various immune cells), as well as IL27-mediated signaling events. EBI3 interacts with genes IL27, IL12A, CANX, MDF1, and SMAD3. It has been associated and researched in sepsis, asthma, carcinoma, Chron's disease, b-cell lymphomas, ulcerative colitis, Hodgkin disease, lymphoma, ebv infections, and glomerulonephritis.

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Gene Symbol

EBI3

Additional EBI3 Products

Product Documents for Recombinant Human EBI3 Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human EBI3 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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