Recombinant Human EEF1D His Protein

Name: Recombinant Human EEF1D His Protein
Brand: Novus Biologicals
SKU: NBP1-51028

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51028

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all EEF1D Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-281 of Human EEF1D

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MATNFLAHEK IWFDKFKYDD AERRFYEQMN GPVAGASRQE NGASVILRDI ARARENIQKS LAGSSGPGAS SGTSGDHGEL VVRIASLEVE NQSLRGVVQE LQQAISKLEA RLNVLEKSSP GHRATAPQTQ HVSPMRQVEP PAKKPATPAE DDEDDDIDLF GSDNEEEDKE AAQLREERLR QYAEKKAKKP ALVAKSSILL DVKPWDDETD MAQLEACVRS IQLDGLVWGA SKLVPVGYGI RKLQIQCVVE DDKVGTDLLE EEITKFEEHV QSVDIAAFNK I

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EEF1D His Protein

SDS-PAGE: Recombinant Human EEF1D His Protein [NBP1-51028]

SDS-Page: Recombinant Human EEF1D Protein [NBP1-51028] - 15 % SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-51028

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEF1D

Eukaryotic translation elongation factor 1 delta, also known as EEF1D, is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Overexpression of EEF1D is associated with oesophageal carcinoma and may adversely affect the outcome of medulloblastomas. Recombinant human EEF1D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

Antigen NY-CO-4, EF-1D, EF-1-delta, EF1DFP1047, elongation factor 1-delta, eukaryotic translation elongation factor 1 delta (guanine nucleotide exchangeprotein), FLJ20897, guanine nucleotide exchange protein

Gene Symbol

EEF1D

Additional EEF1D Products

Product Documents for Recombinant Human EEF1D His Protein

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COA

Product Specific Notices for Recombinant Human EEF1D His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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