Is the His-tag on #NBP1-78833 EIF4A3 protein on the n-term or the c-term?

The His tag on this protein is located on the N-terminus.

For NBP1-78833, EIF4A3 protein, the product information says to store at 4C short term or aliquot and store at -20C long term. Due to the 30% glycerol in storage buffer, it does not freeze at -20C. Most enzymes require storage at -80C for stability. Do you have data to suggest that the protein is stable at -20C? Do you have data to suggest that it is NOT stable for storage at -80C with freeze/thaw?

You can store long term at -80C. You are quite right that the addition of glycerol will protect the protein from freeze-thaw damage but it will not freeze at -20C. Storage at -80C for long term in aliquots will be better.

Recombinant Human EIF4A3 His Protein

Name: Recombinant Human EIF4A3 His Protein
Brand: Novus Biologicals
SKU: NBP1-78833

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78833

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all EIF4A3 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-411 of Human EIF4A3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMATTAT MATSGSARKR LLKEEDMTKV EFETSEEVDV TPTFDTMGLR EDLLRGIYAY GFEKPSAIQQ RAIKQIIKGR DVIAQSQSGT GKTATFSISV LQCLDIQVRE TQALILAPTR ELAVQIQKGL LALGDYMNVQ CHACIGGTNV GEDIRKLDYG QHVVAGTPGR VFDMIRRRSL RTRAIKMLVL DEADEMLNKG FKEQIYDVYR YLPPATQVVL ISATLPHEIL EMTNKFMTDP IRILVKRDEL TLEGIKQFFV AVEREEWKFD TLCDLYDTLT ITQAVIFCNT KRKVDWLTEK MREANFTVSS MHGDMPQKER ESIMKEFRSG ASRVLISTDV WARGLDVPQV SLIINYDLPN NRELYIHRIG RSGRYGRKGV AINFVKNDDI RILRDIEQYY STQIDEMPMN VADLI

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

49.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EIF4A3 His Protein

SDS-PAGE: Recombinant Human EIF4A3 His Protein [NBP1-78833]

SDS-Page: Recombinant Human EIF4A3 Protein [NBP1-78833] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-78833

Formulation	20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 30% glycerol, 200 mM NaCl
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EIF4A3

EIF4A3 (Eukaryotic initiation factor 4A-III) belongs to the DEAD box helicase family and eIF4A subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. EIF4A3 is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. Recombinant human EIF4A3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DDX48, DEAD (Asp-Glu-Ala-Asp) box polypeptide 48, DEAD box protein 48, DKFZp686O16189, EC 3.6.1, EC 3.6.4.13, eIF4AIII, eIF-4A-III, eIF4A-III, eukaryotic initiation factor 4A-III, Eukaryotic initiation factor 4A-like NUK-34, eukaryotic translation initiation factor 4A, Eukaryotic translation initiation factor 4A isoform 3, eukaryotic translation initiation factor 4A3, hNMP 265, KIAA0111eukaryotic translation initiation factor 4A, isoform 3, MGC10862, NMP 265, NMP265, Nuclear matrix protein 265, NUK34

Gene Symbol

EIF4A3

Additional EIF4A3 Products

Product Documents for Recombinant Human EIF4A3 His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human EIF4A3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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