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Recombinant Human Endothelial Lipase His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-51639

Novus Biologicals, part of Bio-Techne
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NBP2-51639-0.1mg
NBP2-51639-0.5mg

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 21-340 of Human Endothelial Lipase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSPVPFGP EGRLEDKLHK PKATQTEVKP SVRFNLRTSK DPEHEGCYLS VGHSQPLEDC SFNMTAKTFF IIHGWTMSGI FENWLHKLVS ALHTREKDAN VVVVDWLPLA HQLYTDAVNN TRVVGHSIAR MLDWLQEKDD FSLGNVHLIG YSLGAHVAGY AGNFVKGTVG RITGLDPAGP MFEGADIHKR LSPDDADFVD VLHTYTRSFG LSIGIQMPVG HIDIYPNGGD FQPGCGLNDV LGSIAYGTIT EVVKCEHERA VHLFVDSLVN QDKPSFAFQC TDSNRFKKGI CLSCRKNRCN SIGYNAKKMR NKRNSKMYLK TRA

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Endothelial Lipase His Protein

SDS-PAGE: Recombinant Human Endothelial Lipase His Protein [NBP2-51639]

SDS-PAGE: Recombinant Human Endothelial Lipase His Protein [NBP2-51639]

SDS-Page: Recombinant Human Endothelial Lipase Protein [NBP2-51639]

Formulation, Preparation and Storage

NBP2-51639
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endothelial Lipase

Endothelial lipase (EL) is a novel member of the lipoprotein lipase gene family. Endothelial lipase is synthesized by endothelial cells and functions at the site of synthesis. EL most likely plays a physiological role in modulating lipoprotein metabolism. Overexpressed EL has been shown to reduce HDL cholesterol levels, whereas an Endothelial lipase deficiency appears to be effective in elevating HDL cholesterol levels in animals.

Alternate Names

EC 3.1.1, EC 3.1.1.3, EDLendothelial lipase, ELlipoprotein lipase H, Endothelial cell-derived lipase, lipase, endothelial, PRO719

Gene Symbol

LIPG

Additional Endothelial Lipase Products

Product Documents for Recombinant Human Endothelial Lipase His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human Endothelial Lipase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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