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FBXO42 Recombinant Protein Antigen

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84711PEP

Novus Biologicals, part of Bio-Techne
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NBP1-84711PEP

Key Product Details

Source

E. coli

Tag

N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

Conjugate

Unconjugated

Applications

Antibody Competition

Product Specifications

Description

A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human FBXO42.

Source: E. coli

Amino Acid Sequence: HSLPPIARRLGHHPPQSLNVGKPLYQSMNCKPMQMYVLDIKDTKEKGRVKWKVFNSSSVVGPPETSLHTVVQGRGELIIFGGLMDKKQNVKY

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Purity

>80% by SDS-PAGE and Coomassie blue staining

Predicted Molecular Mass

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications

Antibody Competition (10 - 100 molar excess)

Application Notes

This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-84711.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Protein / Peptide Type

Recombinant Protein Antigen

Formulation, Preparation and Storage

NBP1-84711PEP
Formulation PBS and 1M Urea, pH 7.4.
Preservative No Preservative
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: FBXO42

F-box only protein 42 (FBXO42) is a substrate recognition component of the SKP1-CUL1-F-box protein (SCF) type E3 ubiquitin ligase complex. The FBXO42 protein recognizes p53/TP53; which promotes its ubiquitination and degradation. FBXO42 is a member of the F-box protein family. Members of this family are characterized by an approximately 40-amino acid F-box motif. F-box proteins are also known to interact with SKP1 and other ubiquitination targets through their interaction domains.

Alternate Names

F-box only protein 42, F-box protein 42, Fbx42, JFK, Just one F-box and Kelch domain-containing protein, KIAA1332FBX42

Gene Symbol

FBXO42

Additional FBXO42 Products

Product Documents for FBXO42 Recombinant Protein Antigen

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FBXO42 Recombinant Protein Antigen

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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