Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06718
Key Product Details
Source
E. coli
Tag
Strep (N-Term)
Conjugate
Unconjugated
Applications
ELISA, Flow Cytometry, SDS-PAGE
Product Specifications
Description
A recombinant protein with a N-Terminal Strep-tag corresponding to 175 amino acids of Felis domesticus 1.0101
Source: E.coli
Accession Numbers: NP_001041618.1 (Chain 1), NP_001041619.1 (Chain 2)
Store product aliquots at -20C to -70C. Use stored aliquots within 6 months.
Purity
>95%, by HPLC
Predicted Molecular Mass
19.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein
![Flow Cytometry: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718]](https://resources.bio-techne.com/images/products/Recombinant-Feline-Felis-domesticus-1-0101-Strep-N-Term-Protein-Flow-Cytometry-NBP3-06718-img0003.jpg "Flow Cytometry: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718]")
Flow Cytometry: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718]
Flow Cytometry: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718] - Flow cytometry dot-plot staining pattern of Felis domesticus 1.0101 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (NB100-77913) FITC and anti-human CD203c PE antibodies.![ELISA: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718]](https://resources.bio-techne.com/images/products/Recombinant-Feline-Felis-domesticus-1-0101-Strep-N-Term-Protein-ELISA-NBP3-06718-img0001.jpg "ELISA: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718]")
ELISA: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718]
ELISA: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718] - ELISA (enzyme-linked immunosorbent assay) test was designed to prove the bond between the coated target recombinant allergen Felis domesticus 1.0101 and allergen-specific human plasma IgG4 antibodies of Felis domesticus positive donor. A measurable signal was subsequently generated by the addition of biotin labeled anti-human IgG4 (detection) antibody, Streptavidin-HRP and substrate solution (TMB). The intensity of the signal is proportional to the amount of coated Felis domesticus 1.0101.![SDS-PAGE: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718]](https://resources.bio-techne.com/images/products/Recombinant-Feline-Felis-domesticus-1-0101-Strep-N-Term-Protein-SDS-Page-NBP3-06718-img0002.jpg "SDS-PAGE: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718]")
SDS-PAGE: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718]
SDS-Page: Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein [NBP3-06718] - Recombinant allergen Felis domesticus 1.0101 purity verification. 5 ug of Felis domesticus 1.0101 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.Formulation, Preparation and Storage
NBP3-06718
| Preparation Method | The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification. |
| Formulation | Lyophilized from a 0.22 um filtered solution in Tris buffer (100 mM Tris, 150 mM NaCl, pH 8) |
| Preservative | No Preservative |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Reconstitution | Reconstitute with sterile deionized water with or without azide. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
Background: Felis domesticus 1.0101
Alternate Names
rFel d 1
Gene Symbol
LOC677877
Additional Felis domesticus 1.0101 Products
Product Documents for Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein
Product Specific Notices for Recombinant Feline Felis domesticus 1.0101 Strep (N-Term) Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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