Recombinant Mouse FGF-17 Protein

Name: Recombinant Mouse FGF-17 Protein
Brand: Novus Biologicals
SKU: NBP2-61334

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61334

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Product Datasheet / COA

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

View all FGF-17 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 194 amino acids corresponding to FGF-17 Source: E. coli

Amino Acid Sequence: TQGENHPSPN FNQYVRDQGA MTDQLSRRQI REYQLYSRTS GKHVQVTGRR ISATAEDGNK FAKLIVETDT FGSRVRIKGA ESEKYICMNK RGKLIGKPSG KSKDCVFTEI VLENNYTAFQ NARHEGWFMA FTRQGRPRQA SRSRQNQREA HFIKRLYQGQ LPFPNHAERQ KQFEFVGSAP TRRTKRTRRP QSQT

Purity

>98%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of FGF-17 as determined by LAL method.

Predicted Molecular Mass

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

FGF-17 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 IU/mg in the presence of 10 ug/ml of heparin.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-61334

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 700 mM NaCl, with 0.02 % Tween-20.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile PBS to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: FGF-17

The FGF17 gene codes for a fibroblast growth factor 17 protein that in isoform 1 is 216 amino acids long at 24 kDA and in isoform 2 is 205 amino acids long at 23 kDA. This gene is a member of the fibroblast growth factor (FGF) family so is thought to participate in nervous system development and function, specifically in embryonic development as well as a signaling molecule in the induction and patterning of the embryonic brain. FGF14 is involved in regulation of actin cytoskeleton, MAPK signaling, signaling by FGFR and FGFR mutants, as well as downstream signal transduction. It interacts with genes EGFR, FGFR1, FGFR2, FGFR3, and FGFR4. FGF14 is associated with pancreatitis, breast cancer, leukemia, melanoma, and adenocarcinoma.

Long Name

Fibroblast Growth Factor 17

Alternate Names

FGF17

Gene Symbol