Recombinant Mouse Flt-3 Ligand/FLT3L His Protein

Name: Recombinant Mouse Flt-3 Ligand/FLT3L His Protein
Brand: Novus Biologicals
SKU: NBP2-52182

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52182

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
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Key Product Details

Source

Baculovirus

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Flt-3 Ligand/FLT3L Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 27-189 of Mouse Flt-3 Ligand

Source:Baculovirus

Amino Acid Sequence: GTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQHHHHHH

Purity

>85%, by SDS-PAGE

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Predicted Molecular Mass

19.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Flt-3 Ligand/FLT3L His Protein

SDS-PAGE: Recombinant Mouse Flt-3 Ligand/FLT3L His Protein [NBP2-52182]

SDS-Page: Recombinant Mouse Flt-3 Ligand Protein [NBP2-52182] - 15% SDS Page (3 ug)

Formulation, Preparation and Storage

NBP2-52182

Formulation	PBS (pH 7.4), 10% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation. Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation.

Long Name

fms-like Tyrosine Kinase 3 Ligand

Alternate Names

FLG3L, Flt3L, FLT3LG

Gene Symbol

FLT3LG

Additional Flt-3 Ligand/FLT3L Products

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COA

Product Specific Notices for Recombinant Mouse Flt-3 Ligand/FLT3L His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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