Recombinant Mouse GAPDH His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52622
Key Product Details
Source
E. coli
Tag
His
Conjugate
Unconjugated
Applications
Enzyme Activity, SDS-PAGE
Product Specifications
Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-333 of Mouse GAPDH
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVKVGVN GFGRIGRLVT RAAICSGKVE IVAINDPFID LNYMVYMFQY DSTHGKFNGT VKAENGKLVI NGKPITIFQE RDPTNIKWGE AGAEYVVEST GVFTTMEKAG AHLKGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIVSN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGAAQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTPN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTED QVVSCDFNSN SHSSTFDAGA GIALNDNFVK LISWYDNEYG YSNRVVDLMA YMASKE
Purity
>95%, by SDS-PAGE
Predicted Molecular Mass
38.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Specific activity is > 40 units/mg, and is defined as theamount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Mouse GAPDH His Protein
![SDS-PAGE: Recombinant Mouse GAPDH His Protein [NBP2-52622]](https://resources.bio-techne.com/images/products/Recombinant-Mouse-GAPDH-Protein-SDS-Page-NBP2-52622-img0002.jpg "SDS-PAGE: Recombinant Mouse GAPDH His Protein [NBP2-52622]")
SDS-PAGE: Recombinant Mouse GAPDH His Protein [NBP2-52622]
SDS-Page: Recombinant Mouse GAPDH Protein [NBP2-52622] - Theoretical molecular weight - 38.2 kDaFormulation, Preparation and Storage
NBP2-52622
| Formulation | PBS (pH 7.4), 20% glycerol, 1 mM DTT |
| Preservative | No Preservative |
| Concentration | 0.25 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: GAPDH
References
1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908
2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079
3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169
4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139
5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968
Long Name
Glyceraldehyde-3-phosphate Dehydrogenase
Alternate Names
G3PDH
Entrez Gene IDs
NP_032110 (Mouse)
Gene Symbol
GAPDH
Additional GAPDH Products
Product Documents for Recombinant Mouse GAPDH His Protein
Product Specific Notices for Recombinant Mouse GAPDH His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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