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Recombinant Human GATC His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23079

Novus Biologicals, part of Bio-Techne
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NBP2-23079

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-136 of Human GATC

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GATC His Protein

SDS-PAGE: Recombinant Human GATC His Protein [NBP2-23079]

SDS-PAGE: Recombinant Human GATC His Protein [NBP2-23079]

SDS-Page: GATC Protein [NBP2-23079]

Formulation, Preparation and Storage

NBP2-23079
Formulation 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 30% glycerol, 1 mM DTT
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GATC

GATC allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). This protein is subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. Recombinant human GATC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

FLJ37000, gatC, glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial), MGC129938,15E1.2gatC-like protein, Protein 15E1.2

Gene Symbol

GATC

Additional GATC Products

Product Documents for Recombinant Human GATC His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human GATC His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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