Recombinant Human GCDH His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50838

Novus Biologicals, part of Bio-Techne
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NBP1-50838-0.25mg
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all GCDH Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 45-438 of Human GCDH

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRPEFDWQDP LVLEEQLTTD EILIRDTFRT YCQERLMPRI LLANRNEVFH REIISEMGEL GVLGPTIKGY GCAGVSSVAY GLLARELERV DSGYRSAMSV QSSLVMHPIY AYGSEEQRQK YLPQLAKGEL LGCFGLTEPN SGSDPSSMET RAHYNSSNKS YTLNGTKTWI TNSPMADLFV VWARCEDGCI RGFLLEKGMR GLSAPRIQGK FSLRASATGM IIMDGVEVPE ENVLPGASSL GGPFGCLNNA RYGIAWGVLG ASEFCLHTAR QYALDRMQFG VPLARNQLIQ KKLADMLTEI TLGLHACLQL GRLKDQDKAA PEMVSLLKRN NCGKALDIAR QARDMLGGNG ISDEYHVIRH AMNLEAVNTY EGTHDIHALI LGRAITGIQA FTASK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

45.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GCDH His Protein

SDS-PAGE: Recombinant Human GCDH His Protein [NBP1-50838]

SDS-PAGE: Recombinant Human GCDH His Protein [NBP1-50838]

SDS-Page: Recombinant Human GCDH His Protein [NBP1-50838] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-50838
Formulation 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 5mM DTT, 200 mM NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GCDH

Glutaryl-CoA dehydrogenase(GCDH) belongs to the acyl-CoA dehydrogenase family. GCDH catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. This Protein uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. Recombinant GCDH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

ACAD5, EC 1.3.99, EC 1.3.99.7, GCD, glutaryl-CoA dehydrogenase, glutaryl-CoA dehydrogenase, mitochondrial, glutaryl-Coenzyme A dehydrogenase

Gene Symbol

GCDH

Additional GCDH Products

Product Documents for Recombinant Human GCDH His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human GCDH His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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