Recombinant Mouse GDF-7/BMP-12 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61338
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity, Western Blot
Product Specifications
Description
A homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains corresponding to GDF-7/BMP-12 Source: E. coli
Uniprot ID: P43029
Amino Acid Sequence: TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of GDF-7/BMP-12 as determined by LAL method.
Predicted Molecular Mass
29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2000 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Mouse GDF-7/BMP-12 Protein
![SDS-PAGE: Recombinant Mouse GDF-7/BMP-12 Protein [NBP2-61338]](https://resources.bio-techne.com/images/products/Recombinant-Mouse-GDF-7-BMP-12-Protein-SDS-Page-NBP2-61338-img0002.jpg "SDS-PAGE: Recombinant Mouse GDF-7/BMP-12 Protein [NBP2-61338]")
SDS-PAGE: Recombinant Mouse GDF-7/BMP-12 Protein [NBP2-61338]
SDS-Page: Recombinant Mouse GDF-7/BMP-12 Protein [NBP2-61338]Formulation, Preparation and Storage
NBP2-61338
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: GDF-7/BMP-12
Long Name
Growth Differentiation Factor 7
Alternate Names
BMP-12, GDF7
Gene Symbol
GDF7
Additional GDF-7/BMP-12 Products
Product Documents for Recombinant Mouse GDF-7/BMP-12 Protein
Product Specific Notices for Recombinant Mouse GDF-7/BMP-12 Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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