Recombinant Human Glucose 6 phosphate isomerase His Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52232
Key Product Details
Source
E. coli
Tag
His
Conjugate
Unconjugated
Applications
Enzyme Activity, SDS-PAGE
Product Specifications
Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-558 of Human Glucose 6 phosphate isomerase
Source: E.coli
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ
Purity
>95%, by SDS-PAGE
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Predicted Molecular Mass
65.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Specific activity is > 400 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will convert 1.0 umole of D-Fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 37C.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human Glucose 6 phosphate isomerase His Protein
![SDS-PAGE: Recombinant Human Glucose 6 phosphate isomerase His Protein [NBP2-52232]](https://resources.bio-techne.com/images/products/Recombinant-Human-Glucose-6-phosphate-isomerase-Protein-SDS-Page-NBP2-52232-img0002.jpg "SDS-PAGE: Recombinant Human Glucose 6 phosphate isomerase His Protein [NBP2-52232]")
SDS-PAGE: Recombinant Human Glucose 6 phosphate isomerase His Protein [NBP2-52232]
SDS-Page: Recombinant Human Glucose 6 phosphate isomerase Protein [NBP2-52232]Formulation, Preparation and Storage
NBP2-52232
| Formulation | 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Background: Glucose 6 phosphate isomerase
Alternate Names
AMFGNPI, Autocrine motility factor, DKFZp686C13233, EC 5.3.1.9, glucose phosphate isomerase, glucose-6-phosphate isomerase, hexose monophosphate isomerase, hexosephosphate isomerase, Neuroleukin, NLKSA36, oxoisomerase, PGI, PHI, Phosphoglucose isomerase, phosphohexomutase, Phosphohexose isomerase, phosphosaccharomutase, SA-36, Sperm antigen 36, sperm antigen-36
Gene Symbol
GPI
Additional Glucose 6 phosphate isomerase Products
Product Documents for Recombinant Human Glucose 6 phosphate isomerase His Protein
Product Specific Notices for Recombinant Human Glucose 6 phosphate isomerase His Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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