Recombinant Human Glutathione Peroxidase 3/GPX3 His Protein

Name: Recombinant Human Glutathione Peroxidase 3/GPX3 His Protein
Brand: Novus Biologicals
SKU: NBP1-99090

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99090

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Glutathione Peroxidase 3/GPX3 Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 21-226 of Human Glutathione Peroxidase 3/GPX3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQSRGQEKSK MDCHGGISGT IYEYGALTID GEEYIPFKQY AGKYVLFVNV ASYCGLTGQY IELNALQEEL APFGLVILGF PCNQFGKQEP GENSEILPTL KYVRPGGGFV PNFQLFEKGD VNGEKEQKFY TFLKNSCPPT SELLGTSDRL FWEPMKVHDI RWNFEKFLVG PDGIPIMRWH HRTTVSNVKM DILSYMRRQA ALGVKRK

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

25.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Glutathione Peroxidase 3/GPX3 His Protein

SDS-PAGE: Recombinant Human Glutathione Peroxidase 3/GPX3 His Protein [NBP1-99090]

SDS-Page: Recombinant Human Glutathione Peroxidase 3/GPX3 Protein [NBP1-99090]

Formulation, Preparation and Storage

NBP1-99090

Formulation	20 mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15 M NaCl, 1 mM DTT, 50 mM Imidazole
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glutathione Peroxidase 3/GPX3

GPX3, also known as glutathione peroxidase 3, belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. GPX3 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX3(U73C) protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography

Alternate Names

GPX-P, GPX3

Gene Symbol

GPX3

Additional Glutathione Peroxidase 3/GPX3 Products

Product Documents for Recombinant Human Glutathione Peroxidase 3/GPX3 His Protein

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COA

Product Specific Notices for Recombinant Human Glutathione Peroxidase 3/GPX3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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