Recombinant Human GMDS His Protein

Name: Recombinant Human GMDS His Protein
Brand: Novus Biologicals
SKU: NBP1-72521

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72521

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all GMDS Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-372 of Human GMDS

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAHAPARCPS ARGSGDGEMG KPRNVALITG ITGQDGSYLA EFLLEKGYEV HGIVRRSSSF NTGRIEHLYK NPQAHIEGNM KLHYGDLTDS TCLVKIINEV KPTEIYNLGA QSHVKISFDL AEYTADVDGV GTLRLLDAVK TCGLINSVKF YQASTSELYG KVQEIPQKET TPFYPRSPYG AAKLYAYWIV VNFREAYNLF AVNGILFNHE SPRRGANFVT RKISRSVAKI YLGQLECFSL GNLDAKRDWG HAKDYVEAMW LMLQNDEPED FVIATGEVHS VREFVEKSFL HIGKTIVWEG KNENEVGRCK ETGKVHVTVD LKYYRPTEVD FLQGDCTKAK QKLNWKPRVA FDELVREMVH ADVELMRTNP NA

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

44.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human GMDS His Protein

SDS-PAGE: Recombinant Human GMDS His Protein [NBP1-72521]

SDS-Page: Recombinant Human GMDS Protein [NBP1-72521] - 15% SDS-PAGE (3ug)

Formulation, Preparation and Storage

NBP1-72521

Formulation	20 mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1 M NaCl, 1 mM DTT, 0.1 mM PMSF
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GMDS

GMDS, also known as SDR3E1, belongs to the GDP-mannose 4,6-dehydratase family. This protein utilizes NADP as a cofactor to catalyze the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose. Mutations in the gene encoding GMDS are involved in resistance to TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)-induced apoptosis. Recombinant human GMDS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

EC 4.2.1.47, GDP-D-mannose dehydratase, GDP-mannose 4,6-dehydratase, GMDGDP-mannose 4,6 dehydratase, SDR3E1, short chain dehydrogenase/reductase family 3E, member 1

Gene Symbol

GMDS

Additional GMDS Products

Product Documents for Recombinant Human GMDS His Protein

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COA

Product Specific Notices for Recombinant Human GMDS His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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